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A high-throughput microfluidic platform for single cell screening

We have developed a massively parallel microchemostat platform capable of growing 1152 bacterial or yeast strains in parallel. The platform is integrated to allow automated image acquisition with high spatio-temporal resolution. With this platform we conducted the first proteome wide survey of protein expression dynamics in S. cerevisiae in response to MMS induced DNA damage. We also applied the platform to screening of a M. smegmatis knock-out library (the model organism for M. tuberculosis) for mutants which are hyper- or hypo -sensitive to an antibiotic yielding potential drug targets and basic insights into bacterial persistence.

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